Seawater carbonate chemistry and RNA:DNA ratios, SOD activity, catalase activity, and immunoblots of HSP 70 in Mytilus edulis

DOI

The interactive effects of multiple stressors associated with global climate change will expose marine organisms to physiological challenges potentially exceeding their current abilities to acclimatize. As a result the ecological effects of the rapidly increasing magnitude, and variability, of environmental change combined with the unknown physiological capacity to acclimatize (= phenotypic plasticity) or genetically adapt, remains uncertain for many marine organisms. In the Gulf of Maine (GOM), the rocky intertidal harbors the blue mussel, Mytilus edulis, an important ecosystem engineer in these communities. Using mussel collections in the Southwest GOM from different tidal heights and a common garden experiment, mussels show significant, phenotypically plastic, effects of tidal height in multiple parameters related to metabolic capacity and stress tolerance. When these mussels are then experimentally exposed to the independent and interactive effects of thermal stress and ocean acidification, several biomarkers of stress (e.g., oxidative stress, HSP70, protein synthesis) are elevated in treatments with higher temperatures, but when combined with lower pH consistent with future predictions these markers show evidence of metabolic depression. In marine ecosystems, exposure to ocean acidification has been hypothesized as a factor that would narrow the thermal window of physiological tolerance for many invertebrates such as marine mussels. The data presented here provide evidence supporting that hypothesis in blue mussels from the GOM, an ecosystem facing one of the greatest rates of change in the marine environment.

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-3-1.

Identifier
DOI https://doi.org/10.1594/PANGAEA.941567
Related Identifier https://doi.org/10.1002/lno.10326
Related Identifier https://cran.r-project.org/web/packages/seacarb/index.html
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.941567
Provenance
Creator Lesser, Michael P
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Contributor Yang, Yan
Publication Year 2016
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Representation
Language English
Resource Type Dataset
Format text/tab-separated-values
Size 284 data points
Discipline Earth System Research
Spatial Coverage (-69.400 LON, 42.967 LAT)