This study aims to examine 1) whether E. diaphana from the GBR, which harbour B. minutum as its homologous symbiont, can form a stable symbiosis with heterologous wild-type and heat-evolved Cladocopium C1acro, and 2) the nutritional consequences of the respective Cladocopium C1acro associations with specific focus on central carbon metabolism. Since sugars are a key form in which Symbiodiniaceae translocate fixed carbon to the anemone host, particular focus is given to sugar-related metabolites.