Flow Cytometry Data and Fluorescence Microscopy Images of V79 and LN18 Cells Exposed to 250 kVp X Rays

DOI

V79 and LN18 were exposed to 250 kVp x rays at doses from 0 to 3 Gy. The cells were then prepared for either flow cytometry or fluorescence microscopy 1.5 doubling times later.

In the case of the microscopy, the cells were blocked from dividing using cytochalisin b. Imaging was done with a Nikon Eclispe CiS microscope using a 10x objective. The resulting images are contained in the data set.

Flow cytometry was done using a double DNA stain using SYTOX Green and Ethidium Monoazide (EMA) using a BD FACSCanto II. The generated FCS files are included in the dataset.

This dataset was the result of a PhD, titled "MiNIMUS: A Model to Predict the Formation and Numbers of Micronuclei in Cells.", available open access via Surrey Research Insight (http://epubs.surrey.ac.uk).

Identifier
DOI https://doi.org/10.23728/b2share.f54a1c6505e441909e4c54e0621a9284
Source https://b2share.eudat.eu/records/f54a1c6505e441909e4c54e0621a9284
Metadata Access https://b2share.eudat.eu/api/oai2d?verb=GetRecord&metadataPrefix=eudatcore&identifier=oai:b2share.eudat.eu:b2rec/f54a1c6505e441909e4c54e0621a9284
Provenance
Creator Adam Cole
Publisher EUDAT B2SHARE; http://b2share.eudat.eu
Publication Year 2015
Rights Creative Commons Attribution-NonCommercial-ShareAlike (CC-BY-NC-SA); info:eu-repo/semantics/openAccess
OpenAccess true
Contact adam.cole09(at)gmail.com
Representation
Language English
Resource Type Other
Format zip
Size 2.4 GB; 3 files
Discipline Other