Sequencing of Cassette Expressing Human OTC in Yeast

Sequencing of coding variants in yeast yOTC expression cassette. yOTC encodes the human OTC protein, using a codon-optimized derivative of the human OTC cDNA sequence. Derivatives of yOTC were introduced into haploid yeast strains so that each encoded a version of human OTC with one amino acid changed. Reads were aligned to Genbank ON872185.1 positions 2047-3061.

Identifier
Source https://data.blue-cloud.org/search-details?step=~01202EFA7C43AA45764DF66F7F25D343BAAAF7B4EB4
Metadata Access https://data.blue-cloud.org/api/collections/02EFA7C43AA45764DF66F7F25D343BAAAF7B4EB4
Provenance
Instrument NextSeq 500; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Contributor PNRI
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Coverage Begin 2023-04-04T00:00:00Z
Temporal Coverage End 2023-04-07T00:00:00Z