Adsorption of Fab and Fc from monoclonal antibody at the oil/water interface

DOI

Following our previous study of adsorption of globular lysozyme and BSA, we continue to explore how different globular proteins adsorb at the oil/water interface by neutron reflection. Inter is preferred because we have built technical capability on this instrument. Fab and Fc are the constituents of a full mAb and we propose to study their adsorption before the whole mAb. Our specific aims are to (a) determine the actual thickness and volume fraction of Fab and Fc and (b) the effect of pH on the interfacial distribution. The results will enable us to compare their extent of distributions into the oil region with other globular proteins at the interface. The results will enable us to answer if all the globular proteins unfold and produce characteristic segment distributions into the oil region. This work will form the basis for us to study the adsorption of different antibodies.

Identifier
DOI https://doi.org/10.5286/ISIS.E.82892478
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/82892478
Provenance
Creator Mr Ruiheng Li; Mr Zhiming Lu; Dr Zongyi Li; Dr Mario Campana; Mr Sean Ruane; Dr John Webster; Professor Jian Lu
Publisher ISIS Neutron and Muon Source
Publication Year 2019
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Natural Sciences; Physics
Temporal Coverage Begin 2016-10-03T08:00:00Z
Temporal Coverage End 2016-10-06T08:00:00Z