Yeast and fast-growing human tumor cells share metabolic similarities in that both cells use fermentation of glucose for energy and both are highly sensitive to the glucose analog 2-deoxyglucose. In this study, we selected for mutations that conferred 2-deoxyglucose resistance to the budding yeast Saccharomyces cerevisiae. Whole genome sequencing was used to identify mutations and RNAseq was used to characterize changes in the transcriptome.