Yeast cell instance-segmentation dataset of the paper "An Instance Segmentation Dataset of Yeast Cells in Microstructures" [EMBC, 2023]. https://arxiv.org/abs/2304.07597 We present and publicly release (under MIT license) a dataset of yeast (Saccharomyces cerevisiae) cells in microstructures with instance segmentation annotations. The dataset is comprised of 493 densely annotated brightfield microscopy images (instance segmentation) of different TLFM experiments with different microstructures (traps). Please refer to our GitHub repository or to the paper for more information. Project page: https://christophreich1996.github.io/yeast_in_microstructures_dataset/ Code (data loading & evaluation): https://github.com/ChristophReich1996/Yeast-in-Microstructures-Dataset