During the last 2 decades, VRE</p><p>have become significant nosocomial pathogens worldwide,</p><p>mainly due to their adaptability in hospital environments and the</p><p>limited treatment options. Nine types of glycopeptide resistance</p><p>determinants (vanA, vanB, vanC, vanD, vanE, vanG, vanL, vanM,</p><p>and vanN) have been reported and well characterized on the basis</p><p>of phenotypic and genotypic criteria.</p><p>We first reported the vanM gene in a vancomycin-resistant</p><p>Enterococcus faecium (VREm) clinical isolate from a teaching hospital</p><p>in Shanghai in 2006. Subsequently, only a single study</p><p>from Singapore has reported vanM-type VRE isolates.Therefore we performed whole genome sequencing of three Enterococcus faecium isolates to validate the way of vanM transduction using bioinformatics.