Microenvironmental Confinement Drives Density-Dependent Neuron-Like Cell Proliferation and Contact-Dependent Neurite Outgrowth [data]

DOI

This dataset accompanies the manuscript entitled "Microenvironmental Confinement Drives Density-Dependent Neuron-Like Cell Proliferation and Contact-Dependent Neurite Outgrowth," and contains microscopy images (.tiff) and associated raw tracing data (.csv, *.swc, .traces) collected using Fiji's Simple Neurite Tracer plugin. Data was generated from PC12 cells cultured within 3D-printed confinement structures, acquired using an Olympus IX-81 inverted microscope. Python scripts (.py) are provided to replicate analyses such as data filtering, statistical testing, and figure generation described in the manuscript. The dataset is structured by experiment IDs and days in vitro (DIV), facilitating detailed examination of neuron-like cell proliferation and neurite growth patterns under varied confinement conditions.

Identifier
DOI https://doi.org/10.11588/DATA/5GDKXK
Metadata Access https://heidata.uni-heidelberg.de/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=doi:10.11588/DATA/5GDKXK
Provenance
Creator Casale, Stephen ORCID logo; Selhuber-Unkel, Christine ORCID logo; Cavalcanti-Adam, Elisabetta Ada ORCID logo; Schmidt, Målin; Taheri, Fereydoon
Publisher heiDATA
Contributor Selhuber-Unkel, Christine
Publication Year 2025
Funding Reference Health + Life Science Alliance Heidelberg Mannheim ; Flagship Initiative "Engineering Molecular Systems" ; Excellence Strategy 2082/1-390761711 (3D Matter Made to Order) ; Carl Zeiss Foundation ; Volkswagen Foundation “Life?” (Az. 96733) ; German Research Foundation SPP SE 1801/5-1, (2 303 731) ; Proof of Concept Grant VASCUGRAFT, no. 899701 ; INST 35/1643-1 FUGG
Rights CC BY 4.0; info:eu-repo/semantics/restrictedAccess; http://creativecommons.org/licenses/by/4.0
OpenAccess false
Contact Selhuber-Unkel, Christine (Heidelberg University, IMSEAM, Matter to Life)
Representation
Resource Type Dataset
Format application/zip; text/plain
Size 62601; 1631421917; 11771
Version 1.1
Discipline Basic Biological and Medical Research; Biology; Cell Biology; Construction Engineering and Architecture; Engineering; Engineering Sciences; Life Sciences; Medicine