Seawater carbonate chemistry and the microbiome of the octocoral Lobophytum pauciflorum

DOI

Bacteria associated with marine invertebrates are thought to have a range of important roles that benefit the host including production of compounds that may exclude pathogenic microorganisms and recycling of essential nutrients. This study characterised the microbiome of a gonochoric octocoral, Lobophytum pauciflorum, and investigated whether either sex or environmental stresses influenced the diversity of the associated microbiome through amplicon profiling of the bacterial 16S rRNA gene. Sequences affiliated to Spirochaetaceae and Endozoicimonaceae dominated the microbiome of L. pauciflorum, representing 43% and 21% of the community, respectively. Among the dominant class affiliations, no sex-specific differences were detected, though unassigned sequences were at a 2-fold higher relative abundance in samples from female individuals than from males. These potentially novel sequences contributed to observed differences between sexes as detected by a multivariate analysis at the OTU level. Exposing L. pauciflorum fragments to increased temperature (31°C), decreased pH (7.9) or both stressors simultaneously for 12 days did not significantly alter the microbial community, indicating that the soft coral microbiome is relatively resilient to short-term environmental stress.

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2018-09-04.

Supplement to: Wessels, Wiebke; Sprungala, Susanne; Watson, Sue-Ann; Miller, David J; Bourne, David G (2017): The microbiome of the octocoral Lobophytum pauciflorum: minor differences between sexes and resilience to short-term stress. FEMS Microbiology Ecology, 93(5)

Identifier
DOI https://doi.org/10.1594/PANGAEA.893397
Related Identifier IsSupplementTo https://doi.org/10.1093/femsec/fix013
Related Identifier IsDocumentedBy https://cran.r-project.org/package=seacarb
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.893397
Provenance
Creator Wessels, Wiebke; Sprungala, Susanne; Watson, Sue-Ann ORCID logo; Miller, David J ORCID logo; Bourne, David G
Publisher PANGAEA
Contributor Yang, Yan
Publication Year 2017
Rights Creative Commons Attribution 3.0 Unported; https://creativecommons.org/licenses/by/3.0/
OpenAccess true
Representation
Resource Type Supplementary Dataset; Dataset
Format text/tab-separated-values
Size 1036 data points
Discipline Earth System Research
Spatial Coverage (146.480 LON, -18.560 LAT)
Temporal Coverage Begin 2013-08-01T00:00:00Z
Temporal Coverage End 2014-10-30T00:00:00Z