In total, 24 amphibian species are known from Kakamega Forest, Western Kenya. They belong to the families Bufonidae (2 spp.), Dicroglossidae (1 sp.), Arthroleptidae (2 spp.), Hyperoliidae (8 spp.), Ranidae (1 sp.), Pipidae (1 sp.), Phrynobatrachidae (3 spp.), Pyxicephalidae (1 sp.), and Ptychadenidae (5 spp.). During the BIOTA project, one frog species (Leptopelis mackay Köhler et al. 2006) was new described. The species is endemic to Kakamega Forest and its vicinities. Two species represent new records for Kakamega Forest.