Transcriptomic analysis of Aspergillus fumigatus and Klebsiella pneumoniae grown as single and mixed biofilms. Overall design: RNA Sequencing was performed of Aspergillus fumigatus and Klebsiella pneumoniae grown as single and mixed biofilms for 24 hours at 37 °C in YPD medium. For mixed biofilms, Aspergillus fumigatus was pre-cultured for 12 hours to allow germination prior addition of Klebsiella pneumoniae. Cultures were harvested and three biological replicates were subjected for RNA isolation and sequencing library preparation.