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Detection of ? in RNA oligonucleotides and in tRNAs from S. cerevisiae strain...
We have engineered an RT-active DNA polymerase variant called RT-KTq I614Y that produces error RT-signatures specific for pseudouridine (?) without prior chemical treatment of... -
DEtail-seq profiled meiotic DSBs in Saccharomyces cerevisiae
SPO11-promoted DNA double-strand breaks (DSBs) formation is a crucial step for meiotic recombination, and it is indispensable to detect the broken DNA ends accurately for... -
Genome-Wide CRISPR Screens for Toxoplasma Gondii Genes Related to Artemisinin...
We used CRISPR to generate pools of T. gondii mutants in the presence of dihydroartemisinin to identify genes that confer increased or decreased sensitivity to this drug.... -
Deep mutational scanning quantifies DNA binding and predicts clinical outcome...
Nonsense and missense mutations in the transcription factor PAX6 cause a wide range of eye development defects, including aniridia, microphthalmia and coloboma. To understand... -
Nanopore sequencing improves construction of customized CRISPR-based gene act...
CRISPR-based screening has emerged as a powerful tool for identifying new gene targets for desired cellular phenotypes. The construction of gRNA pools largely determines library... -
HIV-1 RNA structure and heterogeneity analysis
The goal of this set of experiments is to determine the structure of HIV-1 NL4-3 and NHG RNA structure using a novel algorithm that allows for the identification of multiple RNA... -
Direct RNA sequencing on an array of nanopores
In this work we compare a novel technology – nanopore direct RNA sequencing – to nanopore cDNA sequencing and sequencing-by-synthesis. We use messenger RNA isolated from...
