-
Determining the fitness of hemizygous hybrid yeast
In this study we found the genome-wide effect of hemizygosity of a Saccharomyces cerevisiae x Saccharomyces uvarum hybrid by matng the deletion collection to S. uvarum. We then... -
Rapid Sequencing of 100kb input S cerevisiae DNA
Rapid Sequencing of 100kb input S cerevisiae DNA -
Protein chaperones elicit a caloric restriction-like response to extend repli...
The involvement of protein chaperones in aging is intriguing and their potential contribution has, so far, been attributed solely to their central role in proteostasis. Here we... -
Sporulation: TAO3 replacement strains
From a previous study (Deutschbauer 2005), 3 single nucleotide variants responsible for the sporulation efficiency of SK1 were identified. Here, we characterized the precise... -
Rec114 ChIP-seq
To segregate accurately during meiosis, homologous chromosomes in most species must recombine. Very small chromosomes would risk missegregation if recombination were randomly... -
Identification of exceptionally potent adenosine deaminases RNA editors from ...
The most abundant type of RNA editing in Metazoa is the deamination of adenosine to inosine, A to I, catalyzed by ADAR enzymes, double strand specific deaminases. Inosines are... -
Mutational_Signatures_in_the_YKO_collection
This project is being carried out through a Strategic Award from the Wellcome Trust to Steve Jackson, Mike Stratton, Peter Campbell, Serena Nik-Zainal and others. This grant is... -
QuantSeq/3' targeted seq supporting study Probing 3’UTRs as modular regulator...
The goal of the study is to analyze the function of 3'-UTR motifs in the non-native context. Several 3'-UTR motifs predicted to have stabilizing or destabilizing effects on mRNA... -
Context dependent function of transcriptional regulator Rap1 in gene silencin...
As part of a study on the function of Rap1 at HML and MAT in Saccharomyces cerevisiae, we performed an Anchor Away experiment on Rap1, inducibly depleting it from the nucleus... -
RiboDoc: a Docker-based package for ribosome profiling analysis
Ribosome profiling (RiboSeq) has emerged as a powerful technique for studying the genome-wide regulation of translation in various cells. Several steps in the biological... -
Yeast sequences
Sequences were performed as part of research concerning (maintenance of) genetic variation -
Pronounced and Extensive Microtubule Defects in a Saccharomyces cerevisiae DI...
The recently proposed exozyme hypothesis posits that subunits of the RNA processing exosome assemble into structurally distinct protein complexes that function in disparate... -
Genome-wide targets of conserved histone chaperone Asf1 in budding yeast
Asf1, through its histone chaperone activity, helps chromatin closing/opening during DNA replication, repair, recombination and transcription. Despite extensive research on... -
Sequencing data for paper: "Engineering a Model Cell for Rational Tuning of G...
Nanopore sequencing data to confirm a series of CRISPR edits made in paper "Engineering a Model Cell for Rational Tuning of GPCR Signaling" -
Genetic and pharmacological evidence for kinetic competition between alternat...
mPAT approach using an anchored oligo-dT (TV12VN) primer to determine changes to 3'UTR length in yeast upon cordycepin treatment and new transcription using 4-thiouracil (4tU)... -
Intrinsically disordered regions of transcription factor encode multiple func...
Intrinsically disordered regions (IDRs) are abundant within eukaryotic proteins, but their sequence-function relationship remains poorly understood. IDRs of transcription... -
Sequencing of Cassette Expressing Human OTC in Yeast
Sequencing of coding variants in yeast yOTC expression cassette. yOTC encodes the human OTC protein, using a codon-optimized derivative of the human OTC cDNA sequence.... -
Genetic dissection of chromatin regulators reveals the INO80 chromatin remode...
We performed RNA-seq in wild-type yeast, a strain lacking INO80, and a strain lacking ARP5, central components of the INO80 chromatin remodelling complex, treated with rapamycin... -
Spliceosome profiling visualizes the operations of a dynamic RNP in vivo at n...
Tools to understand how the spliceosome functions in vivo have lagged behind advances in its structural biology. We describe methods to globally profile spliceosome-bound... -
Young Genes are More Responsive to Environmental Stress than Ancient Genes in...
S.cer (strain Cen.PK113-7D), K.marx (strain CBS6556), and Y.lipo (strain W29) transcriptional profiles were compared after chemostat growth Transcriptome