This repository is for the microscopy data presented in the manuscript CP12 controls ribulose 1,5 bisphosphate recycling and carbon acquisition in Chlamydomonas reinhardtii Cassy Gérard, Régine Lebrun, Christophe Verthuy, Hugo Le Guenno, Artemis Kosta, Florence Guérard, Kwang Suk Chang, Luisana Avilan, Bertrand Gakière, EonSeon Jin, Stephen C. Maberly, Brigitte Gontero, Hélène Launay

This include electron microscopy images in format .dm4

For full details about culture conditions and culture strains, refer to the article. Three strains were analysed: Delta-CP12 (deletion of CP12 gene), Delta-CP12 complemented with the CP12 gene and WT cells (strain CC 4349). Cells were pelleted, high pressure frozen, freeze substituted and embedded in LR White resin (Hard Grade), according to (McDonald, 2014). Ultrathin sections were prepared with an ultracryomicrotome (EM UC7 Leica), and immunogold labelling was performed (O’Toole, 2010). Primary antibodies RuBisCO raised against the large subunit were used to detect RuBisCO, and were coupled to secondary Aurion Protein G conjugated to 10 nm gold particles (nanogold particles). The samples were analysed using a Tecnai 200 kV electron microscope (Thermofisher Scientific), and digital acquisitions were made with a numeric camera (Oneview, Gatan).

Refer to Readme file for the architecture of the data (two biological replicates, three strains and two culture conditions).