Evolutionary and functional analysis of DNA methyltransferases in micro-eukaryotes: Insights from the model diatom Phaeodactylum tricornutum [Bisulfite-Seq]

DNA methylation has been associated with transcriptional repression of transposable elements. Here we describe the first DNA methylation deficient cell line of a diatom by generating DNMT5:KOs in Phaeodactylum tricornutum. These data show the extend of the loss of DNA methylation in DNMT5:KOs compared to the reference strain. All DNA methylation is lost in DNMT5:KOs. Overall design: 3 conditions - 2 independent DNMT5:KOs -> DNMT5:KO line M23 DNMT5:KO line M25 + 1 reference strain "WT" Pt18.6. 1 replicate per condition.

Identifier
Source https://data.blue-cloud.org/search-details?step=~01207543EE566D4A31C761D071C01FF7C213F1B9E22
Metadata Access https://data.blue-cloud.org/api/collections/07543EE566D4A31C761D071C01FF7C213F1B9E22
Provenance
Instrument 544; 308
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2026
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Point 2023-03-19T00:00:00Z