In the current dataset is presented the characterization of wastewaters from cotton textile industry, as well as the characterization of the carbohydrate oxidases, N-acetylglucosamine oxidase (NagOX) and cellobiose oxidase (COX), in terms of pH and temperature profile, substrate recognition and pH stability. Additionally, the hydrogen peroxide production in wastewater using both enzymes is presented, evaluating the operational modality.

Data of H2O2 production by two carbohydrate oxidases, N-acetylglucosamine oxidase (NagOX) and cellobiose oxidase (COX), using wastewater from the cotton textile industry as feedstock

Description of the methods used to collect and generate the data: The determination of enzymatic acitivity was conducted using Britton-Robinson buffer 50 mM. Cellobiose 20 mM and Glucose 50 mM were used as substrate. the method used 0.1 4-aminopyridine and 1 mM of Sodium 3,5-dichloro-2-hydroxy-benzenesulfonate, in presence of 4 AU of peroxidase from horseradish. The determination of carbohydrate profile was conducted in HPLC-IR, using a Luna Omega column (150mm x 4.6 x 3um) supplied by Phenomenex. The mobile phase was acetonitrile (65% v/v), 1 mL/min of flow rate at 30°C. * Total suggar content was determined using Benedict's method, using copper sulfate at high temperature and alkali conditions. *Hydrogen peroxide (H2O2) was determined using vanadium pentoxide in presence of sulfuric acid (H2SO4) at 454 nm. * Stability test were performed in 2mL eppendorf. * Enzymatic pretreatment of wastewaters was perfomed with 0.5% of commercial blend of glucoamyalses. Information about instruments, calibration and standards: * Varian Cary 50 Bio UV-visible spectrophotometer (Agilent) * Varian Cary 3 UV-visible spectrophotometer (Agilent) * SPECTROstar Nano (BMG LABTECH) photometer * French press (OneShot, Constant System) * Fast Protein Liquid Chromatography (FPLC) in ÄKTA Pure (GE Healthcare®, Chicago, IL, USA) Environmental or experimental conditions: Hydrogen peroxide production was performed in wastewaters at pH 8.0 and 50°C using NagOX and pH 6.0 and 30°C using COX.