Seawater carbonate chemistry and expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera during experiments, 2012, supplement to: Nakamura, Masoko; Morita, Masaya; Kurihara, Haruko; Mitarai, Satoshi (2012): Expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera under prospective acidified conditions over the next several decades. Biology Open, 1(2), 75-81

DOI

Ocean acidification is an ongoing threat for marine organisms due to the increasing atmospheric CO2 concentration. Seawater acidification has a serious impact on physiologic processes in marine organisms at all life stages. On the other hand, potential tolerance to external pH changes has been reported in coral larvae. Information about the possible mechanisms underlying such tolerance responses, however, is scarce. In the present study, we examined the effects of acidified seawater on the larvae of Acropora digitifera at the molecular level. We targeted two heat shock proteins, Hsp70 and Hsp90, and a heat shock transcription factor, Hsf1, because of their importance in stress responses and in early life developmental stages. Coral larvae were maintained under the ambient and elevated CO2 conditions that are expected to occur within next 100 years, and then we evaluated the expression of hsps and hsf1 by quantitative real-time polymerase chain reaction (PCR). Expression levels of these molecules significantly differed among target genes, but they did not change significantly between CO2conditions. These findings indicate that the expression of hsps is not changed due to external pH changes, and suggest that tolerance to acidified seawater in coral larvae may not be related to hsp expression.

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).

Identifier
DOI https://doi.org/10.1594/PANGAEA.779704
Related Identifier https://doi.org/10.1242/bio.2011036
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.779704
Provenance
Creator Nakamura, Masoko; Morita, Masaya; Kurihara, Haruko; Mitarai, Satoshi
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Contributor Nisumaa, Anne-Marin
Publication Year 2012
Funding Reference Seventh Framework Programme, 211384; Sixth Framework Programme, 511106
Rights Creative Commons Attribution 3.0 Unported; https://creativecommons.org/licenses/by/3.0/
OpenAccess true
Representation
Language English
Resource Type Supplementary Dataset; Dataset
Format text/tab-separated-values
Size 256 data points
Discipline Earth System Research