During the cruise of EMB238 at several stations in Fehmarn Belt area macrofauna samples were collected within the framework of DAM pilot mission project, MGF Baltic Sea, aiming to investigate the impact of bottom trawling fishing in marine protected areas of the German Exclusive Economic Zone (EEZ). The data contains the abundance, biomass and species number. The water depths of all stations was around 24 metres. Samples were obtained using van Veen grab, sieved with 1 mm sieve and fixed with formalin on board. Sorting with microscopy, taxonomic identification, counting and weighing of the species took place in the laboratory.