Our previous work has revealed that under high Cu levels,</p><p>the A. fumigatus transcription factor AceA is activated, inducing the expression of the</p><p>copper exporter CrpA to expel excess Cu.</p><p>To identify additional elements in Cu resistance, we evolved A. fumigatus wild-type</p><p>and mutant daceA or dcrpA strains to increasing Cu concentrations. Sequencing of</p><p>the resultant resistant strains identified both shared and unique evolutionary pathways</p><p>to resistance.