SANS characterization of hybrid vesicles lipid/polybutadiene-polyethyleneoxide (DPPC/OB) for the insertion of membrane proteins

DOI

Amphiphilic polymers are biocompatible, tunable and versatile macromolecules used in biotechnology and drug delivery. Similarly to the phospholipids that constitute the membrane of cells, they form bilayers in the form of vesicles (polymersomes or PS). PS can be used, for instance, to deliver drugs and release them according to certain stimuli (temperature, pH...). The properties of PS can be tuned by modifying the chemical structure of the polymer itself or by mixing them with lipid such as DPPC. We use mixtures of DPPC and PEGylated polymer in the form of hybrid vesicles to achieve the reconstitution of membrane proteins (MPs). Such MPs will be tested for directionality and functionality. Together with other techniques, SANS will help to characterize better the lipid/polymer bilayer. Such information will allow to tune the components to obtain the appropriate matrix to host MPs.

Identifier
DOI https://doi.org/10.5286/ISIS.E.82352394
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/82352394
Provenance
Creator Dr Gianluca Bello; Miss Francesca Cavallini; Dr Sarah Rogers
Publisher ISIS Neutron and Muon Source
Publication Year 2019
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2016-09-14T08:00:00Z
Temporal Coverage End 2016-09-16T08:00:00Z