The major purpose of National BioResource Project (NBRP) is to collect, preserve, and provide bioresources (such as experimental animals and plants) that are essential experimental materials for life sciences research. The project also aims to improve the bioresources by increasing the value of bioresources through enriching genome information and developing fundamental technologies for preservation and other necessary procedures, in order to meet to the current scientific demands. NIG had been responsible for the systematic collection, storage, and distribution of Escherichia coli and Bacillus subtilis strains. In this project, to enrich the genome information of our E. coli and B. subtilis stocks, we carried out the whole genome sequencing.