pyMarAI: nnU-Net-based Tumor Spheroids Auto Delineation

DOI

Collection of neural network models for automatic image segmentation of microscopic tumor spheroids. Intended to be used with nnU-Net deep-learning framework. Trained and tested on a total of microscopic images of mouse pheochromocytoma (MPC) tumor cells.

In addition to the trained network model, a PyQt5-based graphical user interface tool is provided. This tool provides a complete pipeline for handling microscopic spheroid image data, running deep-learning–based delineation, and curating results for continuous model improvement.

For installation and usage instructions, please visit https://github.com/hzdr-MedImaging/pyMarAI

Please cite nnU-Net and the respective paper when using pyMarAI.

List of available model types:

pyMarAI-1.0.0-ecat.zip: nnUNetv2 ready network (for ECAT7)
pyMarAI-1.0.0-nifti.zip: nnUNetv2 ready network (for NIFTI)
Identifier
DOI https://doi.org/10.14278/rodare.4199
Related Identifier IsIdenticalTo https://www.hzdr.de/publications/Publ-42498
Related Identifier IsReferencedBy https://www.hzdr.de/publications/Publ-42497
Related Identifier IsSupplementTo https://github.com/hzdr-MedImaging/pyMarAI
Related Identifier IsPartOf https://doi.org/10.14278/rodare.4198
Related Identifier IsPartOf https://rodare.hzdr.de/communities/health
Related Identifier IsPartOf https://rodare.hzdr.de/communities/hzdr
Related Identifier IsPartOf https://rodare.hzdr.de/communities/pet-center
Related Identifier IsPartOf https://rodare.hzdr.de/communities/rodare
Related Identifier IsPartOf https://rodare.hzdr.de/communities/zrt
Metadata Access https://rodare.hzdr.de/oai2d?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:rodare.hzdr.de:4199
Provenance
Creator Maus, Jens ORCID logo; Nitschke, Janina; Nikulin, Pavel ORCID logo; Hofheinz, Frank ORCID logo; Barth, Mareike; Lemm, Sandy ORCID logo; Richter, Lena; Pietzsch, Jens ORCID logo; Braune, Anja ORCID logo; Ullrich, Martin ORCID logo
Publisher Rodare
Publication Year 2026
Rights Creative Commons Attribution Share Alike 4.0 International; Open Access; https://creativecommons.org/licenses/by-sa/4.0/legalcode; info:eu-repo/semantics/openAccess
OpenAccess true
Contact https://rodare.hzdr.de/support
Representation
Resource Type Software
Version 1.0.0
Discipline Life Sciences; Natural Sciences; Engineering Sciences