This is a dataset of continuous dissolved gas measurements made by a laser spectroscopy based gas analyzers coupled to a gas extraction module, and plumbed directly into the seawater supply line of the RV Sally Ride. This data was collected as part of the PUPCYCLE II cruise, which took place in the California Upwelling system (CalUS) (38-44°N, and 121-126°W from May 28, 2023 to June 12, 2023). Underway measurements were calibrated using discrete dissolved gas samples (doi:10.1594/PANGAEA.987238). The goal of the study was to understand controls on the biogeochemical cycling of gases in the CalUS and quantify sea-air fluxes of the gases, particularly the understudied gas of CH4 and N2O. In addition to the dissolved gas measurements, there is also measurements of stable carbon isotopes (del13C) of methane and carbon dioxide, temperature, oxygen, fluorescence and salinity.