This dataset contains stable isotope ratios of C, N and S in several macrofaunal taxa sampled at the Vestbrona carbonate field, a methane seep are off the coast of Norway. All details about sampling and analyses can be found in the article "Lack of detectable chemosynthesis at a sponge dominated subarctic methane seep" by Melina Sinner, Wei Li Hong, Loïc N. Michel, Sunil Vadakkepuliyambatta, Jochen Knies and Arunima Sen (currently under revision for Frontiers in Marine Sciences). A full term description of the terms used in the dataset is available at https://doi.org/10.17882/76595.