This study aimed to understand the differences in gene expression when comparing Aspergillus fumigatus swollen conidia that overexpress an artifical Bir1 construct (strain published in Shlezinger et. al 2017) versus a parental control strain in RPMI + 0.5ug/mL voriconazole. The purpose of this experiment was to understand what basal expression differences may drive a decreased susceptibility to hydrogen peroxide upon subsequent treatment (not tested in this submission). The main results show that there is a significant upregulation of mitochondrial-related genes, included aconitate hydratase. These differences may account for increased susrvival upon cellular stressors in this strain background. Overall design: RNA-seq profiling of swollen Conidia representing various genotypes: A. fumigatus wt strain, ATCC46645 +Histone H2A:: mRFP fusion, ATCC46645 +Histone H2A:: mRFP fusion + Bir1OX, ATCC46645 +Histone H2A:: mRFP fusion + Bir1OX-N 1-446aa.