This table contains physical, climatological, and isotope data. All lakes were open lakes. The slope was calculated according to methods/ranges proposed by the United States Department of Agriculture so that each watershed is described with a unitless slope characteristic, with slope gradient classes above 45 indicating steep drainage basins. The land uses were calculated from Chilean vegetative data (methods section) and values represent the proportion (%) of the catchment that is covered with this type of land use. Then, refer it as % snow or glaciers, that means that the zone is covered permanently with this land use. MAT and MAP, are mean annual air temperature and precipitation calculated from a monthly reanalysis product (CR2MET) with a 0.05° horizontal resolution considering the period from 1979 to 2016, and extracting data from the nearest grid point to the lake positions where we collected surface water. All samples were taken during austral spring/summer 2017 to 2019.