Interaction of human dihydroorotate dehydrogenase with ubiquinone in model lipid bilayers

DOI

Human dihydroorotate dehydrogenase (DHODH) is a membrane-bound enzyme of clinical interest and a well-validated drug target for e.g. rheumatoid arthritis and multiple sclerosis. The exact way in which DHODH interacts with membrane-bound ubiquinone (coenzyme Q10) and with inhibitors is not known. Our goal is to use investigate the mechanisms by which DHODH interacts with ubiquinone, with lipids and with inhibitors in planar lipid bilayers mimicking the composition of the inner mitochondrial membrane. We have carried out preliminary neutron reflectivity experiments at the ILL to investigate the structure of supported lipid bilayers made from synthetic lipids and yeast membranes containing ubiquinones Q2 and Q10. The aim of this experiment is to study the interaction of the catalytic domain of DHODH with ubiquinones Q2 and Q10 in model lipid bilayers.

Identifier
DOI https://doi.org/10.5286/ISIS.E.99687011
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/99687011
Provenance
Creator Dr Hanna Wacklin-Knecht; Mr Juan Manuel Orozco Rodriguez; Mr Robin Delhom; Dr Wolfgang Knecht; Dr Maxmilian Skoda
Publisher ISIS Neutron and Muon Source
Publication Year 2021
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Chemistry; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering; Medicine; Natural Sciences
Temporal Coverage Begin 2018-12-15T09:00:00Z
Temporal Coverage End 2018-12-18T11:46:47Z