Protection of biological molecules during freezing and lyophilisation is of commercial importance to the pharmaceutical, medical, and food industries. Sugars and surfactants both act as cryoprotectants but the mechanisms of protection are not well understood and likely to be different. We have recently carried out the first direct structural studies of cryoprotection of lactate dehydrogenase (LDH) by sucrose and mannitol using a custom built temperature controlled cell on LOQ at ISIS. This scattering data allow us to demonstrate the protein structure breaks down during the freezing process and to observe differences in this process in different sugar solutions. In this current experiment we propose to use SANS2d to repeat the temperature cycling studies and obtain higher quality data with a wider Q-range that will enable us to analyse the structural transitions that take place.