Tackling antimicrobial resistance: structural study of the Annexin A5/Lipopolysaccharide (LPS) complex

DOI

Antimicrobial resistance is a global epidemic and has occurred due to a widespread antibiotic overconsumption. Gram negative bacteria, such as P. aeruginosa (leading cause of mortality in cystic fibrosis patients) are of particular concern due to the limited permeability of bacterial cell wall to antibiotic drugs. One promising strategy is to increase the permeability of the bacterial outer membrane prior to antibiotic delivery. Annexin A5 is a calcium-dependent phospholipid binding protein that targets phosphatidylserine (PS) and under certain conditions can destabilize membranes. Recently it has been shown to also bind LPS-containing membranes and here we propose to study the interaction of Annexin A5 with PS and LPS using neutron reflectivity in order to better understand the structural requirements to turn Annexin A5 into a potentially effective antimicrobial therapy agent.

Identifier
DOI https://doi.org/10.5286/ISIS.E.58450682
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/58450682
Provenance
Creator Dr Arwel Hughes; Dr Benjamin Davis; Dr Filip Ciesielski; Dr Luke Clifton; Dr Maxmilian Skoda
Publisher ISIS Neutron and Muon Source
Publication Year 2018
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering; Medicine
Temporal Coverage Begin 2015-03-21T09:00:00Z
Temporal Coverage End 2015-03-23T09:00:00Z