Palaeolake Kieshofer Moor near Greifswald (Northeastern Germany) has been the first study site for palaeoecological research in the region, with a first pollen record being presented already in the 1920s. To update the palaeoecological research from the area, this new, high resolution pollen record has been produced. Samples have been taken on a sediment record from the centre of the palaeolake, which has been cored in 2007. The record covers the later part of the Weichselian Lateglacial and the Holocene, with the exception of the past around 1000 years. Pollen samples have been prepared and analysed using standard palynological methods. Additionally, the record is used to validate the application of AI based automatic pollen analysis from the record KM23 from the same site.