ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

We performed single-cell transcriptome analysis (using 10x genomics 3' scRNA-seq v3.1 chemistry) in the sea anemone Nematostella vectensis (Cnidaria). Overall design: Several juvenile polyps were dissociated using ACME method and then single-cells were FACS-sorted before 10x encapsulation and transcriptome capture.

Identifier
Source https://data.blue-cloud.org/search-details?step=~012A0D493A435288C941B1A66E3FF18B3320248BF34
Metadata Access https://data.blue-cloud.org/api/collections/A0D493A435288C941B1A66E3FF18B3320248BF34
Provenance
Instrument Illumina HiSeq 2500; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Contributor Sebe-Pedros lab, Systems Biology, Centre for Genomic Regulation
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Point 2021-04-02T00:00:00Z