Incubation Experiments of DOM with Prokaryotic Community at the NW Mediterranean Sea site MOLA in 2021

DOI

The water samples for the incubation experiment were collected on the RV Néréïs II at the Microbial Observatory Laboratory Arago 42N and 4E on 31 May and 26 August 2021. The parameters include DOC/TDN/DON/TDP/DOP, dissolved hydrolyzable amino acids, fluorescent dissolved organic matter (FDOM), enzyme activity, prokaryote heterotrophic production (PHP), prokaryote and viral abundance. The incubation temperature was temperature +13ºC for both experiments. DOC/TDN was analyzed using High-Temperature Catalytic Oxidation (TOC-VCSH). DIN/TDP/DOP was analyzed using a Technicon Auto Analyzer. Dissolved hydrolyzable amino acids were analyzed using High Performal Liquid Chromatography (HPLC). FDOM was measured on a FP-8500 Spectrofluorimeter (Jasco, Japan). Enzymatic activities were measured using 4-methylumbelliferyl-butyrate (esterase), 4-methylumbelliferyl phosphate for alkaline phosphatase (APase), and L-leucine-7-amido-4-methyl coumarin for leu-aminopeptidase (AMA). PHP was measured using 3h-leucine and the microcentrifuge method. Prokaryote and viral abundance were measured on a flow cytometer (Cytoflex, Beckman Coulter, USA). The purpose of data collection was to understand the seasonal cycling of organic matter and heterotrophic bacteria dynamics within the microbial loop.

Identifier
DOI https://doi.pangaea.de/10.1594/PANGAEA.974747
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.974747
Provenance
Creator Bouchachi, Nawal; Campos, Rebeca; Crispi, Olivier; Marie, Barbara ORCID logo; Paulin, Charles-Hubert; Escoubeyrou, Karine; Colombet, Jonathan; Sime-Ngando, Telesphore; Ortega-Retuerta, Eva
Publisher PANGAEA
Publication Year 2025
Funding Reference Agence Nationale de la Recherche https://doi.org/10.13039/501100001665 Crossref Funder ID ANR-20-CE01-0007 https://anr.fr/Project-ANR-20-CE01-0007 MicroPUMP
Rights Creative Commons Attribution 4.0 International; Data access is restricted (moratorium, sensitive data, license constraints); https://creativecommons.org/licenses/by/4.0/
OpenAccess false
Representation
Resource Type Dataset
Format text/tab-separated-values
Size 1332 data points
Discipline Earth System Research
Spatial Coverage (3.690 LON, 42.454 LAT); Mediterranean Sea
Temporal Coverage Begin 2021-05-31T00:00:00Z
Temporal Coverage End 2021-08-26T00:00:00Z