The data herein were used to evaluate the success of subsoil management practices conducted from 2018 to 2020 at the central field sites (CF1 and CF2) of “Campus Klein Altendorf” (Rheinsberg, Germany). Subsoil management practices include deep loosening with and without the incorporation of biowaste compost and green waste compost, as well as using lucerne as deep rooting pre-crop. Subsoil managements are complemented by a control.

Presented are element concentrations of macronutrients (Ca, K, Mg, P), micronutrients (B, Cu, Fe, Mn, Zn), plant beneficial elements (Al, Co, Na, Ni, Si) and elements of non-nutritional character (Ba, Cd, Cr, Ga, Li, Pb, Rb, Sr, Ti) along with soil pH data, magnesium isotope compositions (δ26Mg), and radiogenic strontium isotope ratios (87Sr/86Sr) of samples covering the soil-plant system of the agrosphere. Soil compartments include depth profiles of Luvisol of the easily exchangeable fraction of the soil (1M NH4OAc extraction), and bulk soil (Li2B4O7 fusion digests and microwave assisted sample digests). Plant compartments include bulk ear, bulk stem, and bulk leaf of winter wheat (Triticum aestivum L.) and spring barley (Hordeum vulgare L.) sampled at flowering and maturity stage. Chemical and isotope composition of bulk aboveground crop (shoot) are also presented. Finally, chemical and isotope compositions of compost and converter lime are provided.