Limestone caves have relatively stable temperatures and humidity throughout the year but due to nutrient and energy limitations are considered to be extreme habitats, unfavorable for development of life .limestone caves are generally isolated, cave-adapted species tend to show a high degree of endemism. Cave has been emerged as a model to study biogeography of bacteria. At the same time, microbes collected from pristine sites like caves that is unexplored and rarely visited by humans are likely belong to novel taxa or strains with huge bio-prospecting potential. Moreover, the analysis of the microbial diversity of unique ecosystems provides better understanding of the role of microhabitat in shaping of microbial diversity.