Invasive fungal infections are increasingly endangered due to environmental disperse of antifungal resistance. In particular, azoles are widely used in agriculture as well, which may enable the azole resistant strains of Aspergillus fumigatus persist and be enriched in the environments. Thus, understandings of environmental dissemination and enrichment of genetic variation of azole resistance in A. fumigatus strains are required for suppressing the resistant strains. Here, we focus on multiple strains of azole-resistant A. fumigatus isolated from a single tulip bulb for sale in Japan.