Confocal cleared L4 aphid head-stylets

DOI

Confocal cleared L4 aphid heads: Aphids were cleared/treated after "Smolla M, Ruchty M, Nagel M, Kleineidam CJ: Clearing pigmented insect cuticle to investigate small insects' organs in situ using confocal laser-scanning microscopy (CLSM). Arthropod structure & development 2014, 43(2):175-181.", and observed on a Zeiss confocal laser scanning microscope (488 nm argon laser source), L4 nymphal instars were placed in scanning chambers with methyl salicylate. Aphids were examined using the LSM 800 CLSM with a 10x dry objective (0.40 NA) and 40s and 100x oil immersion objectives (0.7 and 0.95 NA). Optical sections of whole heads were obtained at z-steps ranging from 1 to 5 µm resulting in an x-y plane at 1024x1024 pixel resolution. Three files are provided: an original .czi Zeiss format stack, and Imaris .ims file with contoured 3D object (Retort organs and neighbouring structures), and an Imaris .tif export.

Identifier
DOI https://doi.org/10.15454/VPUGUG
Metadata Access https://entrepot.recherche.data.gouv.fr/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=doi:10.15454/VPUGUG
Provenance
Creator Rahbe, Yvan ORCID logo; Guschinskaya, Natalia; Ressnikoff ORCID logo
Publisher Recherche Data Gouv
Contributor Rahbe, Yves
Publication Year 2019
Rights etalab 2.0; info:eu-repo/semantics/openAccess; https://spdx.org/licenses/etalab-2.0.html
OpenAccess true
Contact Rahbe, Yves (INRA - Institut National de la Recherche Agronomique)
Representation
Resource Type Image; Dataset
Format application/octet-stream; image/tiff; application/vnd.ms-ims
Size 191685952; 286283592; 437336477
Version 1.0
Discipline Agriculture, Forestry, Horticulture; Life Sciences; Zoology; Plant Science