Abstract: In standing ophthalmic surgery in horses, a retrobulbar block (RNB) is often placed blindly for anesthesia and akinesia. The ultrasound (US)-guided block might have less complications, but both techniques have only been compared once in equine cadavers. This study aimed to compare both techniques for success and complication rates and to analyze the effect of training by US-guidance. Twenty-two equine cadavers were divided into three groups: blind RNBs were performed bilaterally in eight cadavers, US-guided RNBs were performed in seven cadavers bi-laterally, and again blind RNBs were performed in seven cadavers bilaterally after US-guided training. All RNBs were performed by the same two inexperienced operators and a combination of contrast medium (CM; 1.25ml) and methylene blue dye (1.25ml) were injected. Needle posi-tioning in the periorbita and the distance of CM to the optic foramen were assessed using com-puterized tomography (CT). Dye spreading was evaluated dissection. In group 1, 37.5% of the in-jections were in the optimal central position in the periorbita; in group 2, 69.2% and in group 3, 71.4%, showing a trend towards significance between groups 1 and 2 (p= 0.056) and groups 1 and 3 (p=0.069). The mean CM distribution distance was not significantly different between all groups. Group 1 had 18.75% intraocular injections versus 0% and 7.1% in group 2 and group 3 (not significant). US-guidance did not show significant increases in accuracy or decreases in complications. However, the effects on accuracy showed a trend towards significant improve-ment and larger scale follow-up studies could show significant training effects of US-guidance.