Phase behaviour of di and triblock eGFP-copolymer conjugates in aqueous media

DOI

Conjugation of therapeutic proteins to polymers is well established but most methods result in multi-site and multiple conjugation or crosslinking resulting in lack of orientation and loss of activity. Using an enzyme mediated conjugation method we have prepared well-defined protein-polymer conjugates using a model protein, green fluorescent protein (eGFP) with diblock copolymers based on several hydrophilic-hydrophobic biocompatible copolymers. In this proposal, we wish to analyse the copolymers alone and to probe eGFP-copolymer conjugates to get a clearer picture of the influence of the molecular weight on morphologies, phase behaviour and order-disorder transitions.

Identifier
DOI https://doi.org/10.5286/ISIS.E.54816435
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/54816435
Provenance
Creator Dr Gavin Hazell; Mr Yun Jin; Dr Amani El Fagui; Professor Karen Edler; Dr Susanna Piluso; Dr Gabriel Cavalli
Publisher ISIS Neutron and Muon Source
Publication Year 2017
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Chemistry; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering; Natural Sciences
Temporal Coverage Begin 2014-07-15T23:00:00Z
Temporal Coverage End 2014-07-17T23:00:00Z