Investigating the impact of varied dietary protein levels on Litopenaeus Vannamei: an exploration of intestinal microbiota and transcriptome responses. Inappropriate feed protein levels reduce antioxidant levels and digestive enzyme activity and promote pathogen settlement. And it reduces factors in various metabolic pathways responding to the organism through transcriptional regulation. This can lead to stunted growth in shrimp and compromise their immune system. The results of the study provide insights into the protein requirements of the species that could provide valuable insights for advancing feed research.