Self-organized pendulum of inductions between the epiblast and extraembryonic endoderm support post-implantation progression of mouse blastoids

DOI

Embryos form partly by tissue self-organization caused by nonlinear cell interactions. Here, we create a partial mouse embryo model to decipher self-organizing principles underlying the co-development of the epiblast (Epi) and Extraembryonic endoderm (XEn). We activate naive embryonic stem cells to form a late blastocyst Epi/XEn-like niche (3D, hydrogel-free, serum-free). Once established, these two lineages autonomously progress in minimal medium to form an inner pro-amniotic-like cavity surrounded by a polarized Epi, and covered with visceral endoderm (VE)-like cells. We observe that progression occurs through a sequence of reciprocal inductions by which the Epi supports the primitive endoderm (PrE) to produce a basal lamina that subsequently regulates Epi polarization/cavitation, which, in return, channels the transcriptomic progression to Visceral endoderm (VE). Finally, the VE contributes to Epi bifurcation into both anterior- and posterior-like states. Similarly, boosting the formation of PrE-like cells within blastoids supports developmental progression. Overall, we argue that self-organization can arise from a pendulum of lineages inductions propagating overtime.

Identifier
DOI https://doi.org/10.34894/GSOHSD
Metadata Access https://dataverse.nl/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=doi:10.34894/GSOHSD
Provenance
Creator Vrij, Erik ORCID logo; Scholte op Reimer, Yvonne; Roa Fuentes, Laury; Misteli Guerreiro, Isabel; Holzmann, Viktoria; Frias Aldeguer, Javier; Sestini, Giovanni; Koo, Bon-Kyoung; Kind, Jop; van Blitterswijk, Clemens; Rivron, Nicolas ORCID logo
Publisher DataverseNL
Contributor Vrij, Erik; Hebels, Dennie
Publication Year 2022
Rights CC0 1.0; info:eu-repo/semantics/openAccess; http://creativecommons.org/publicdomain/zero/1.0
OpenAccess true
Contact Vrij, Erik (Maastricht University); Hebels, Dennie (Maastricht University)
Representation
Resource Type Dataset
Format application/octet-stream; text/plain
Size 84504576; 84426752; 84606976; 84418560; 53030912; 52957184; 74047488; 73928704; 63492096; 63447040; 74018816; 84533248; 84430848; 74121216; 73945088; 30552064; 30494720; 74006528; 73940992; 53043200; 7544832; 7516160; 12947456; 12922880; 53002240; 52948992; 53051392; 52961280; 63508480; 63438848; 84463616; 63537152; 53006336; 46280704; 46215168; 63614976; 63451136; 42573824; 42459136; 42541056; 42553344; 547
Version 1.0
Discipline Life Sciences; Medicine