The data were obtained from two Interlaboratory comparisons (ILC1 and ILC2) involving six Spanish biodosimetry laboratories dedicated to harmonize and establish a standardized national protocol. Both ILCs were organized by La Fe Hospital in Valencia. Two established techniques were applied among the six participant laboratories: the dicentric chromosome assay (DCA) and the cytokinesis-block micronucleus (CBMN) assay. In the first ILC, blood samples were irradiated at 2.9 Gy (sample A) and 1.4 Gy (sample B). In the second ILC, a 2.63 Gy exposure was delivered, generating a partial-body sample (sample A) and a whole-body sample (sample B), the latter prepared by mixing equal proportions of irradiated and non-irradiated blood. The data include the raw values to obtain the calibration curves using the software Biodose Tools v 3.7.2 for each laboratory, and the aberration distribution for the two evaluated samples (sample A and sample B) for each comparison.

Biodose Tools, 3.7.2

METHODOLOGICAL INFORMATION

1. Description of methods used for collection-generation of data Blood samples from one healthy donor were obtained by venipuncture, collected into heparinized tubes, and irradiated ex vivo. For each ILC, two heparinized tubes containing 3 mL of blood were labelled as Sample A and Sample B and sended to the participant laboratories. Each laboratory cultured lymphocytes from the blood they received following their own protocols and the IAEA and ISO standard recommendations. Two laboratories used a semiautomated method to capture the metaphases that were then analysed manually (counting aberrations), and the remaining laboratories performed the cytogenetic analysis directly under the microscope.

2. Methods for processing the data: Biodose Tools v3.7.2 Can be found freely online at https://www.reneb.net/software/

3. Instrument- or software- specific information needed to interpret the data: OpenOffice Biodose Tools v3.7.2 Can be found freely online at https://www.reneb.net/software/

4. Instruments, calibration and standards information: All samples were irradiated ex vivo in a methacrylate tank with water at 37°C using the TrueBeam HDR accelerator located at the Radiation Oncology and Medical Physics Service of La Fe Hospital (Valencia, Spain). Irradiations were performed using a 6 MV photon beam (6X) with a dose rate of 600 MU/min. The irradiation field at isocentre was 8 × 16 cm², and the samples were positioned at a depth of 9 cm. The source-to-surface distance (SSD) was set to 100 cm minus the depth, resulting in an effective SSD of 91 cm. Exposures were delivered using parallel-opposed fields, with the gantry positioned at 90° and 270°, corresponding to left-inferior (LI) and left-dorsal (LD) orientations. The irradiation was administered at the 100% isodose level.