For almost two years, the Tara schooner travelled 70,000 kilometres in the South Atlantic along the entire South American coastline to Antarctica and off the West African coast as part of the Microbiomes Experiment from the EU-AtlantECO project, which funded 42 research institutions from around the world. The experiments aimed to provide knowledge-based resources for better understanding and management of the Atlantic Ocean and its ecosystem services, to investigate the benefits of the marine microbiome and its interactions with climate and pollution. The towed instrument array (TIA), with 10 CTDs installed at different water depths, was towed by the Tara schooner on two transects in the Amazon Plume Experiment. The TIA recorded pressure, temperature, conductivity and fluorescence or oxygen over a distance of 262 km and 274 km respectively.