This dataset presents bulk organic geochemical data (TOC, TN, d13C, d15N) obtained by EA-IRMS analysis on 16 suspended river sediments from vertical profiles in four of the largest rivers of Chilean Patagonia (44-48°S). The samples were collected in November 2011, i.e., in Spring, when river discharge is the highest. Surface samples (10 cm depth) were taken with a bucket, and deeper samples were collected with a horizontal WaterMark® 5L sampling bottle equipped with a depth/pressure sensor. All the water samples were stored in pre-rinsed I-chem cubitainers and filtered within 48 hours using a pressurized filtration system equipped with a 90 mm diameter PES membrane (pore size 0.22 µm).