We established a bacteria infective intestinal inflammation in turbot (Scophthalmus maximus). And found that ß-glucan could significantly alleviate the phenotype of turbot intestinal inflammation. We performed single cell transcriptome analysis to study bacteria infective intestinal inflammation and the effects of ß-glucan. Furthermore, we revealed that ß-glucan through activates Th17 cells to alleviate intestinal inflammation in turbot. Overall design: single cell transcriptome analysis to study bacteria infective intestinal inflammation and the effects of ß-glucan