Konza Prairie Agroecosystem (KONA) is a terrestrial NEON field site is located in the Flint Hills region of Kansas just 10 km (6 mi.) south of Manhattan, KS. Sampling occurs in a 3.6 km2 (903 acre) area of cultivated crop fields owned and managed by Kansas State University’s Department of Agronomy and Department of Animal Science and Industry. The site is focused on sampling within agricultural systems. The surrounding region consists of grasslands, forests, and agricultural land cover types and includes the largest remaining areas of unplowed tallgrass prairie in North America. KONA is part of the NEON Prairie Peninsula Domain (D06), which stretches from Kansas through Nebraska and Iowa to southern Minnesota, and moves east to encompass Illinois and most of Indiana. The Domain hosts four other NEON field sites: two aquatic and two additional terrestrial, all of which are located in Kansas. KONA is colocated with the KING aquatic field site and just 5 km (~3 miles) west of the KONZ terrestrial site. The two sites provide a contrast between more pristine prairie at KONZ and heavily impacted agricultural land at KONA within the same area. Land use and land use management are key grand challenge themes for this Domain.