This database has been designed to meet the growing need to identify fluorophore contaminants in environmental matrices. It gathers spectral fingerprints for 108 fluorescent micropollutants, including hormones (19), pharmaceuticals (41) and pesticides (48). These spectral fingerprints, covering a wide spectral range, enable contaminants to be effectively distinguished from strongly fluorescing organic matter present in environmental samples. The data were obtained by preparing individual solutions of compounds, measuring the fluorescence spectra, and processing the data to eliminate interferences. Each compound is accompanied by full metadata, and the information is available online. This database constitutes a valuable tool for environmental monitoring, offering a comprehensive resource for the identification and characterization of fluorescent pollutants in environmental matrices.