Establishing a reference cultivation protocol for systematic characterisation of the <i>Halomonas</i> genus

DOI

Cultivation profile of 82 strains of Halomonas species in different culture conditionsHalomonas species were cultured in 96-well plates covered with AerasealTM seal and incubated in CLARIOstar Plus (BMG Labtech). The plates were incubated in different culture conditions at 200 rpm: (A) recommended culture conditions, (B) reference temperature with recommended culture medium, (C) LB medium supplemented with the same salt concentration as recommended culture medium, (D) LB medium supplemented with 8% NaCl (LB80), and (E) LB medium supplemented with 2% NaCl (LB20). Optical density at 600 nm (OD600) was measured during the cultivation. Each condition was performed with 6 replicates (n=6).

Identifier
DOI https://doi.org/10.5522/04/32782530.v1
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Provenance
Creator Thanakarn, Waritthorn; Baumbach, Alexander ORCID logo; Price, Eva; Dikicioglu, Duygu
Publisher University College London UCL
Contributor Figshare
Publication Year 2026
Rights https://creativecommons.org/licenses/by/4.0/; http://purl.org/coar/access_right/c_abf2
OpenAccess true
Contact researchdatarepository(at)ucl.ac.uk
Representation
Language English
Resource Type Dataset
Discipline Life Sciences; Medicine; Medicine and Health; Physiology