Newly identified Aspergillus nidulans PKA targets play a role in carbon-catabolite repression

A transcriptomic assessment of a kinase deletion mutant (?pkaA) and a control strain revealed many cellular activities this kinase is impacting. Studies were conducted in the model filamentous fungi, Aspergillus nidulans¸ under rich growth conditions. PkaA is a known kinase that mediates a wide range of processes including: nutrient sensing, stress responses, regulation of metabolism, as well as development and pathogenicity. Here, fungi were grown in rich media containing glucose. The comparison between the control strain and the deletion mutant transcript levels revealed previously unknown targets, such as the transcription factor CreA, the main repressor responsible for carbon catabolite repression (CCR). Overall design: A control strain and deletion mutant grown in rich media (2 biological replicates and 2 technical replicates) were analyzed to determine unknown functions and targest of PkaA using RNA-seq.

Identifier
Source https://data.blue-cloud.org/search-details?step=~0124FA815BD401F360B6ECD6C75B9B0EBD848D0418C
Metadata Access https://data.blue-cloud.org/api/collections/4FA815BD401F360B6ECD6C75B9B0EBD848D0418C
Provenance
Instrument Illumina Genome Analyzer IIx; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Point 2018-07-06T00:00:00Z