Curvibacter sp. AEP1.3 Transcriptome or Gene expression

Every multicellular organism is associated with a complex microbiota, whose interactions are important for maintenance of physiological homeostasis. In order to investigate these interactions, we isolated a bacteriophage we intended to utilize to manipulate the host-associated microbial community of Hydra vulgaris AEP. During this process we tested the phage's ability to infect and eliminate Curvibacter sp. AEP1.3 in liquid culture, on solid medium and in association with Hydra and found that infectivity varied. To further our understanding of these bacterial states, we compared them via RNA Sequencing and found that they differed significantly in their expression patterns. These findings indicated that Curvibacter transitioned between three different lifestyles, which resulted in varying degrees of susceptibility to bacteriophage infection. This concept could be relevant both for microbiota research and phage therapy.

Identifier
Source https://data.blue-cloud.org/search-details?step=~01204F5AE48F86877032AF6BD3258E9E1FD3EA07E22
Metadata Access https://data.blue-cloud.org/api/collections/04F5AE48F86877032AF6BD3258E9E1FD3EA07E22
Provenance
Instrument Illumina NovaSeq 6000; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Temporal Point 2022-05-01T00:00:00Z